Effective and stable gene transduction in rhesus macaque iPSCs capable of T-lineage differentiation utilizing the piggyBac system.
作者：
Tanaka, Masahiro;Iwamoto, Yoshihiro;Wang, Bo;Imai, Eri;Yoshida, Munehiro;...
作者机构：
Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada
语种：
英文
关键词：
Rhesus macaque,T-cell differentiation,iPSC,piggyBac
期刊：
Regenerative Therapy
ISSN：
2352-3204
年：
2024
卷：
27
页码：
104-111
摘要：
INTRODUCTION: Genetically modified human induced pluripotent stem cell (iPSC)-based regenerative medicine has substantial potential in the treatment of refractory human diseases. Thus, preclinical studies on the safety and efficacy of these products are essential. Non-human primate (NHP) models such as the rhesus macaque are highly similar to humans in terms of size, lifespan, and immune system, rendering them superior models. However, effective gene transduction in rhesus macaque iPSCs (Rh-iPSCs) remains challenging. In this study, we investigated the effective gene transduction into Rh-iPSCs and its effect on differentiation efficiency. METHODS: We established a gene tr...
