作者：
Tanaka, Masahiro;Iwamoto, Yoshihiro;Wang, Bo;Imai, Eri;Yoshida, Munehiro;...
通讯作者：
Shin Kaneko
作者机构：
Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada
通讯机构：
Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
语种：
英文
关键词：
iPSC;Rhesus macaque;piggyBac;T-cell differentiation
期刊：
Regenerative Therapy
ISSN：
2352-3204
年：
2024
卷：
27
页码：
104-111
基金类别：
Cooperative Research Program of the Primate Research Institute, Kyoto University; Cooperative Research Program (Joint Usage/Research Center Program) of the Institute for Frontier Life and Medical Sciences, Kyoto University [2020-B-69
摘要：
Introduction Genetically modified human induced pluripotent stem cell (iPSC)-based regenerative medicine has substantial potential in the treatment of refractory human diseases. Thus, preclinical studies on the safety and efficacy of these products are essential. Non-human primate (NHP) models such as the rhesus macaque are highly similar to humans in terms of size, lifespan, and immune system, rendering them superior models. However, effective gene transduction in rhesus macaque iPSCs (Rh-iPSCs) remains challenging. In this study, we investigated the effective gene transduction into Rh-iPSCs and its effect on differentiation efficiency.
Genetically modified human induced...
